| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bifidobacterium thermacidophilum | TGCTGCTTGTCGACGATGT | TGGCACGCGTCTTGATGT | 60.01 | 59.97 | 282 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Bifidobacterium thermacidophilum | ACAACCAGTCGCAGGACAG | TGGTGTTGCCGTCAAGCA | 59.93 | 60.12 | 249 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Bifidobacterium thermacidophilum | CGAAATCAACGGCAGCCAG | TGGTGTTGCCGTCAAGCA | 59.86 | 60.12 | 221 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Bifidobacterium thermacidophilum | GCTCGAAATCAACGGCAGC | TGGTGTTGCCGTCAAGCA | 60.23 | 60.12 | 224 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Bifidobacterium thermacidophilum | TGTTGTCGGTCGATCGCA | TGGCACGCGTCTTGATGT | 59.35 | 59.97 | 186 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Bifidobacterium thermacidophilum | ACGAAGCACAAGGTGGCA | TCCACGTGATGATGCGCA | 59.81 | 59.74 | 219 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Bifidobacterium thermacidophilum | TCAACGTCACAGCTCGCA | AGGCGATCGCTTCAGGAAC | 59.58 | 60.15 | 190 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Bifidobacterium thermacidophilum | GACAACCAGTCGCAGGACA | TGGTGTTGCCGTCAAGCA | 59.93 | 60.12 | 250 | 137 |
100.00%
|
100.00%
|
| Primer 9 | Bifidobacterium thermacidophilum | AACGCAGTACCCGTCACA | TGGTGTTGCCGTCAAGCA | 58.87 | 60.12 | 271 | 137 |
100.00%
|
100.00%
|
| Primer 10 | Bifidobacterium thermacidophilum | ACAACCAGTCGCAGGACA | TGGTGTTGCCGTCAAGCA | 58.77 | 60.12 | 249 | 137 |
100.00%
|
100.00%
|